Calico Crayfish (Faxonius immunis) were collected in summer 2020 at two locations (stars) in Wild Rice Lake of the Manitowish River watershed, Vilas County, Wisconsin, United States (inset map). Population declines of Rusty Crayfish (Faxonius rusticus) in six regularly monitored lakes of the watershed (blue) from Capelli and Magnuson (1983) through Larson et al. (2019) are presented as plots of male catch per unit effort (CPUE) fitted with locally estimated scatterplot smoothing. Crayfish trapping locations in lakes (circles) from Larson et al. (2019), as well as trapping in streams and rivers of the Manitowish River watershed in summer 2021 (crosses; Table 1), are mapped. Infrequent, low abundance (CPUE < 1) collections of the Northern Clearwater Crayfish (Faxonius propinquus) and Virile Crayfish (Faxonius virilis) for these six lakes are available in Larson et al. (2019) supporting information.