Neighbor joining phylogenetic tree with 2000 bootstrap replicates including sequences of Calico Crayfish (Faxonius immunis) collected from Illinois (IL) or Wisconsin (WI). We include four additional F. immunis sequences from GenBank, as well as two sequences each of Virile Crayfish (Faxonius virilis), Northern Clearwater Crayfish (Faxonius propinquus) and Rusty Crayfish (Faxonius rusticus) as three known Faxonius crayfish species from Vilas County, Wisconsin. We used two sequences of Red Swamp Crayfish (Procambarus clarkii) as an outgroup. Nodes on the tree represent bootstrap support, and GenBank accession numbers are given at node tips. GenBank accessions for new F. immunis sequences are OQ759592–OQ759606.