Research Article |
Corresponding author: Efim D. Pavlov ( p-a-v@nxt.ru ) Corresponding author: Tran Duc Dien ( mrtran_cnvb@yahoo.com ) Corresponding author: Ekaterina V. Ganzha ( evganzha@gmail.com ) Academic editor: Charles Martin
© 2023 Efim D. Pavlov, Tran Duc Dien, Ekaterina V. Ganzha.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Pavlov ED, Dien TD, Ganzha EV (2023) Distribution in the estuary and salinity tolerance of armored catfish (Loricariidae) in Central Vietnam. Aquatic Invasions 18(3): 401-414. https://doi.org/10.3391/ai.2023.18.3.104066
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In the last decade, invasive suckermouth armored catfish Pterygoplichthys spp. spread among many river systems of Vietnam. Extended distribution of armored catfish might be associated with using brackish water in estuaries for fish spread from one river system to another. The first goal of our study was to assess the occurrence of armored catfish in the estuary of the Da Rang River (Phu Yen Province, Vietnam) and their distribution depending on the horizontal salinity gradient (4–25 PSU). Fish were mainly caught by stationary bottom traps in water salinity from 4 PSU to 18 PSU. The second goal of our study was to experimentally evaluate the ability of armored catfish to move and breathe in seawater (33 PSU). Fish moved in horizontal and vertical planes after transfer into seawater during the first 15 minutes. Fish moved around less by the 13th–15th minutes in seawater. Armored catfish moved around more in seawater than in freshwater. The exposure to seawater for 6 minutes led to deterioration of fish breathing. The results of our field and experimental studies established that armored catfish are found and able to move in brackish waters but avoid high salinity water. These facts provide support for the hypothesis of armored catfish invasion through the estuaries and coastlines.
invasive fish, fish spreading, brackish water, water salinity, locomotor activity, breathing
The invasive suckermouth armored catfish genus Pterygoplichthys (Siluriformes: Loricariidae) is widely spread and successfully occupies inland waters of the central Indo-Pacific region (
The hypothesis of armored catfish spreading through the estuaries to the rivers was discussed in Southeastern Mexico (
Salinity tolerance is one of several important physiological features that determine invasion success and the pattern of dispersal of introduced aquatic organisms between freshwater bodies through brackish waters (
The detection of armored catfish in estuaries and observation of their movements could support the estuary hypothesis. For subsequent spreading, fish need to move through the brackish waters with different salinity. To increase chances of successfully spreading and decrease mortality risk, fish need to be able to breathe in high salinity water.
The study aimed to estimate the distribution of armored catfish genus Pterygoplichthys in the estuary of the Da Rang River and experimentally assess the effects of high salinity water on fish’s movements and gill respiration.
The study was conducted from February to March 2022. The object of the study was the suckermouth armored catfish genus Pterygoplichthys (Siluriformes: Loricariidae). Two species of armored catfish identified in South Vietnam: P. disjunctivus and P. pardalis (
The Da Rang River was chosen for the field study. This river is one of the largest river systems in Central Vietnam; its length is 374 km, and the basin area is 13900 km2. The river forms a wide and extended estuary in Phu Yen Province and flows into the Eastern Sea within Tuy Hoa City. The annual tide in the estuary varies from 0.5 m to 1.7 m. The Da Rang River discharge during the flood reaches 2100 m3/s, and the average annual river discharge is 275 m3/s (
We caught armored catfish in the estuary of the Da Rang River to study their distribution in the brackish water. The capture was conducted within 3.2–4.2 km of the Eastern Sea coastline (13°03'53"N, 109°18'33"E). Within this zone, four sampling localities were selected with different levels of water salinity near the sea (Figure
Scheme of sampling localities (black circles) in the estuary of the Da Rang River and the water salinity near the bottom. No 1 – nearest place to the Eastern Sea (3.2 km), No 2 – first median place (3.6 km), No 3 – second median place (3.9 km), No 4 – the distant place from the Eastern Sea (4.2 km) (QGIS 3.26.2). Arrow (→) indicates the water flow direction.
To estimate the water salinity stratification, the water salinity was measured from the bottom to the water surface on the sampling locality No 1 closest (3.2 km) to the sea (13°03'55"N, 109°18'40"E) (Figure
Given that armored catfish is benthivore, we placed sectional net traps on the bottom (
The study was conducted at the Coastal Branch of Joint Vietnam-Russia Tropical Science and Technology Research Center (Nha Trang, Vietnam) in March 2022. For experimental work, freshwater armored catfish were caught in the Dinh River in Khanh Hoa Province (12°29'45"N, 109°07'41"E). Fish from the Dinh River was used because we did not have enough fish for experiments from the Da Rang River. The Dinh River length is 50 km, and the basin area is 985 km². The captured fish were transferred to the laboratory in tanks.
The study was performed on 60 fish with a standard body length 14 ± 0.5 (9–24) cm and a body weight 45 ± 5.5 (9–155) g (here and after before the brackets are the mean value and its error; in the brackets are min and max). In the laboratory, fish were separated equally between two 100L maintenance tanks with a water volume of 35L and water temperature 25–26 °C. The tap freshwater (0 PSU) in a laboratory was conditioned by settling and aeration for 2 weeks in two 2000L basins. The water in the maintenance tanks with fish was aerated and changed once per day. The level of dissolved oxygen in the water was 7.0–7.2 mg/L (Pro Dissolved oxygen meter MW600, Milwaukee, USA). Illumination in the maintenance tanks was natural (through the laboratory windows) and varied during the twenty-four hours from 0.1 Lx to 100 Lx (Lux meter Lutron LX-1102). Fish were fed with Pro’s choice tablets – Bottom feeders (Fwusow Industry, Taiwan) for demersal fish twice per day (at 10:00 and 16:00, GMT+7). The fish started feeding on the tablets three days after the transfer. The cumulative fish mortality did not exceed 5% during the first week of maintenance.
Two series of experiments were conducted. In the first series of experiments, we estimated armored catfish locomotor activity and survival rate under exposure with seawater (33 PSU). In the second series of experiments, we estimated locomotor activity and gill respiration under exposure with freshwater (0 PSU) and seawater (33 PSU). Seawater was used from Nha Trang Bay (12°13'03"N, 109°12'47"E) and was conditioned by settling and aeration for 2 weeks. The level of dissolved oxygen in seawater was 7.0–7.2 mg/L. In both series of experiments, the test chambers were two 15L glass aquaria. We used LED backlight placed outside the aquarium (~100 Lx).
The first series of experiments were carried out on 26 fish with a standard body length 12 ± 0.4 (9–18) cm and a body weight 24 ± 2.7 (9–67) g. Each aquarium was filled with 6L of seawater, and the water level was 0.1 m. Two remotely controlled video cameras SjCam A10 (China) were positioned under each aquaria at a distance of 0.2 m from their bottom. Camera recording was controlled by iPad 2018 (Apple Co., USA). At the beginning of the trial, one fish at a time was randomly transferred into each aquarium from the maintenance tanks (see Suppl. material
The second series of experiments were performed on 26 fish with a standard body length 17 ± 0.7 (12–24) cm and a body weight 62 ± 8.7 (18–155) g. For the second series, one aquarium was filled with 6L of freshwater, and the second was filled with 6L of seawater. For video records, we used two GoPro Hero 8 (GoPro Inc., USA) cameras positioned under each aquarium. One fish at a time from the maintenance tanks was randomly transferred to the aquaria with freshwater and one fish was transferred to seawater. Fish assessed under freshwater and seawater exposure did not differ in body length and weight (Student’s t-test: p = 0.62, p = 0.57, respectively). Water in the test aquaria was changed after each trial, and the aquaria with freshwater and seawater were switched. The duration of the trial was six minutes; timing was based on the first series of experimental results to prevent fish mortality in seawater tests. In total, 13 fish were used in freshwater trials and 13 fish were used in seawater trials; 2.5 hours of video were assessed. After trials, fish were transferred into a recovery tank with freshwater and aeration. We used video records to register the duration of fish locomotor activity and their rises to the water surface in the tests. The gill ventilation rate (fv,) (
Statistical data analysis (Minitab 18.1) was conducted with Student’s t-test and Spearman rank correlation coefficient (rs). The Shapiro-Wilk test was used to assess normality distribution of samples. The individual locomotor activity during each minute of the test was assessed by normalized values using the formula:
fa = ti * 100/T
Where: fa – frequency (%) of locomotor activity of fish during each minute of the test, ti – duration of fish moving during ith minute of the test, T – total time of fish moving during the test.
The distribution of the locomotor activity of fish depending on the time of the test was compared with the χ2 criterion.
The number of fish caught in traps was different between sampling localities and increased with a distance away from the sea (Table
Standard body length and body weight of armored catfish Pterygoplichthys spp. from the Da Rang River estuary.
Sampling localities | Maximum registered water salinity, PSU | SL, cm | W, g | Number of alive/dead fish |
---|---|---|---|---|
No 1 | 25 | – | – | 1/– |
No 2 | 18 | 18 ± 2.0 (16–24) | 90 ± 31.2 (47–182) | 2/2 |
No 3 | 14 | 16 ± 1.8 (13–30) | 71 ± 30.1 (28–310) | 4/5 |
No 4 | 4 | 18 ± 1.8 (11–28) | 109 ± 32.9 (23–300) | 10/– |
Along with armored catfish were also captured: Malabar glassy perchlet Ambassis dussumieri, Ambassis sp., silver barb Barbonymus gonionotus, Glossogobius spp., marble goby Oxyeleotris marmorata, Nile tilapia Oreochromis niloticus, long whiskers catfish Mystus gulio, and bartail flathead Platycephalus indicus.
Armored catfish lay down motionless at the bottom of the maintenance tank with freshwater during daylight. Less frequently, fish were located vertically pressed against the side walls.
In the first series of the experiment, all tested fish were transferred to seawater. After transfer, fish usually moved no longer than first minute of the trial; then they lay at the bottom. During the test, fish alternated periods of locomotor activity and inactivity. On average, 20% of the test time fish moved along the perimeter of the aquaria: both near the bottom and rising to the surface water. Fish movements were often abrupt and with brief stops (2–3 sec). Fish locomotor activity in seawater significantly decreased by 13th–15th minutes of the trial (Student’s t-test: 13–15 minutes versus 1–3 minutes, p = 0.009; 13–15 minutes versus 7–9 minutes, p = 0.005) (Figure
In the second series of the experiment, 77% of armored catfish after transfer into freshwater aquaria did not move during the test (for six minutes). Twenty-three percent of fish moved on average for 3.8 ± 0.69 (3.0–5.2) minutes during the trial. Fish movement in the freshwater was smooth. Under seawater exposure, 69% of fish moved on average for 2.0 ± 0.33 (1.1–4.1) minutes during the trial. The fish movements were abrupt with frequent changes in speed and direction. The timing of the locomotor activity of fish in seawater differed at the first 3 minutes and the last 3 minutes of the trial (χ2 criterion: p = 0.01, n = 9). In the seawater in contrast to freshwater, the posterior end of the fish was raised contrary to the anterior end. Sometimes air bubbles were released from the oral cavity and the gill slits. Both processes were observed in 62% of fish at 4.1 ± 0.42 (2.3–5.4) minutes of the trial and were often accompanied by fish movement towards the water surface to capture air. In seawater, 69% of fish had spasms from the fourth minute of the trial. We registered on average 11 ± 2.9 (1–28) body spasms per fish.
After the fish were transferred into freshwater, the gill ventilation rate (fv) remained stable: 206 ± 3.4 (108–294) breaths/min (Figure
Dynamics of the gill ventilation rate (fv) of armored catfish Pterygoplichthys spp. under freshwater (hollow square, n = 13) and seawater (black square, n = 13) exposure during six minutes of testing. The characters indicate: * – significant differences between the data of three initial timing intervals (the first minute of the test) according to Student’s t-test: * – p < 0.001, ** – p < 0.01, *** – p < 0.05. Standard error (|).
Our results confirmed the occurrence of armored catfish in the brackish waters of the Da Rang River estuary in Central Vietnam. The fact that fish were captured in stationary traps is the evidence of their active movements in the estuary. However, this result does not reflect the distance of movements or movement directions of armored catfish in estuaries. To date, armored catfish were found only in brackish waters (up to 8 PSU) in Southeastern Mexico Rivers (
In the Da Rang River estuary, we often caught armored catfish in places located 3.5 km or more from the sea with water salinity up to 18 PSU. That was consistent with our findings that armored catfish could survive in water salinity up to 12.5‒15.0 PSU for two days (
Water salinity is one of the dynamic factors in estuaries. The water salinity depends on the magnitude and phase of the sea tides, the direction and strength of the wind, as well as the intensity and speed of the water flow in the river (
Fish moved for an average of 20% of 15 minutes of the trial (first series of the experiment). Generally, for the first 12 minutes their locomotor activity did not decrease. This feature could allow armored catfish to leave the increased salinity area in nature. But seawater treatment for 15 minutes negatively affected fish survival in the future: 50% of the fish died in the next twenty-four hours.
Most fish (77%), transferred from a freshwater maintenance tank into freshwater aquaria did not move in the aquaria for six minutes (second experiment series). Their gill ventilation rate did not change noticeably during the test period. Most fish (69%), transferred from the freshwater maintenance tank into the seawater aquaria, moved during the trial both horizontally and to the water surface. Thus, the seawater treatment increased the locomotor activity of armored catfish and could stimulate them to leave the area with the high salinity water. Based on this, we conclude that the armored catfish genus Pterygoplichthys can avoid increased salinity water by horizontal movements and rising to the water surface. Hypothetically, armored catfish movements towards the water surface positively affect their survival in the strong stratification of water salinity in the Da Rang River estuary. More studies needed to prove this.
Significant deceleration or cessation of gill respiration was observed during six minutes’ exposure to seawater. Many fish retained the ability to move despite the breathing deterioration. Sixty-two percent of the fish moved to seawater surface to capture air. According to
Experimental results indicated that the locomotor activity of armored catfish in seawater varied between individual fish in duration and intensity. The success of the survival of armored catfish in water with high salinity is probably determined by individual and species characteristics of navigating in the salinity gradient, which was noted by other researchers (
Fish survival rate under seawater treatment for six minutes exceeded 90%, which means that armored catfish could tolerate water with high salinity for a limited period of time. This limited period of time might be enough to change direction of movement, leave the area with high water salinity and spread through the estuary to other influent rivers. But it might not be enough to move to another river system along the coastline. We propose that some environmental factors could facilitate armored catfish to spread along the coastline: the mouths of rivers are located close to each other where water salinity is lower (due to mixing of freshwater and seawater), fish move along a freshwater plume at the surface which is common in estuarine systems dominated by the river. For example, the water surface salinity varied noticeably from 0.5 PSU to 27.0 PSU in Nha Trang Bay located between two estuaries of the adjacent rivers (Cai River and Be River) (
We discovered the armored catfish in the brackish waters of the Da Rang River. Our experiments demonstrated that the fish genus Pterygoplichthys could tolerate seawater for some period of time with preservation of locomotor activity, despite the deterioration of gill respiration. Our results show that armored catfish have physiological features that allow them to spread through estuary and coastline. Armored catfish could stay and move in the estuary. Fish encounter water with different salinity and could tolerate sudden water salinity increases (33 PSU) for a short period of time. Increased movement in seawater might allow them to leave the area with high salinity water.
We propose that the main obstacle to armored catfish invasion is water salinity above 15‒18 PSU. However, a combination of favorable factors increases the chances of fish spreading not only through the estuaries but also through the coastlines. These factors are closely located adjacent river estuaries, plenty of freshwater in the estuary and the coastline area, a freshwater plume at the surface.
A wide range of physiological adaptations of armored catfish allows them not only to successfully spread to new ecosystems but also compete with aboriginal fish for resources. Risks of the potential impact of armored catfish invasion need to be considered when managing the ecosystem.
Field work and experiments were supported by Coastal Branch of Joint Vietnam - Russia Tropical Science and Technology Research Center (Ecolan 3.2 “Taxonomic diversity, ecology and behavior of freshwater hydrobionts”); video data analysis was supported by Russian Science Foundation No. 19-14-00015.
All authors contributed to the study conception and design. Material preparation and data collection in field were performed by EP and DTD. EG and EP performed experimental work and analyzed data. The first draft of the manuscript was written by EP and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
All experimental procedures with fish were carried out according to the guidelines and following the laws and ethics of Socialist Republic of Vietnam and approved by the ethics committee of the Institute of Ecology and Evolution, Russian Academy of Sciences.
Authors would like to thank the administration and staff of Coastal Branch of Joint Vietnam - Russia Tropical Science and Technology Research Center for their help in organizing field sample collection and for kindly allowing us to use their laboratories and experimental facilities. We are grateful to Prof. A.O. Kasumyan, Prof. J.W. Armbruster, and anonymous reviewers for valuable comments on the manuscript’s text.
Chemical parameters of water on the surface and near the bottom (five meters deep) in the Da Rang River estuary
Data type: table (docx. file)
Scheme of test chambers
Data type: figure (docx. file)